Broom A, Rakotoharisoa RV, Thompson MC, Zarifi N, Nguyen E, Mukhametzhanov N, Liu L, Fraser JS & Chica RA* (2020) Evolution of an Enzyme Conformational Ensemble Guides Design of an Efficient Biocatalyst. bioRxiv, doi:


Damry AM, Mayer MM, Broom A, Goto NK & Chica RA* (2019) Origin of Conformational Dynamics in a Globular Protein. Communications Biology 2, 433.

Chica RA* (2019) Designer Sense-Response Systems. Science 366, 952-953. Invited "Perspective" commentary article.

Parmeggiani F, Rué Casamajo A, Colombo D, Ghezzi MC, Galman JL, Chica RA, Brenna E & Turner NJ* (2019) Biocatalytic Retrosynthesis Approaches to D-(2,4,5-trifluorophenyl)alanine, Key Precursor of the Antidiabetic Sitagliptin. Green Chemistry 21, 4368-4379.

St-Jacques AD, Eyahpaise MEC & Chica RA* (2019) Computational Design of Multisubstrate Enzyme Specificity. ACS Catalysis 9, 5480-5485.

Parmeggiani F, Rué Casamajo A, Walton CJW, Galman JL, Turner NJ & Chica RA* (2019) One-Pot Biocatalytic Synthesis of Substituted D-Tryptophans from Indoles Enabled by an Engineered Aminotransferase. ACS Catalysis 9, 3482–3486.


Chica RA* (2018) Designer Proteins Activate Fluorescence. Nature 561, 471-472. Invited "News and Views" commentary article.

Walton CJW, Thiebaut F, Brunzelle JS, Couture JF* & Chica RA* (2018) Structural Determinants of the Stereoinverting Activity of Pseudomonas stutzeri D-Phenylglycine Aminotransferase. Biochemistry 57, 5437-5446.

Wang CY, Chang PM, Ary ML, Allen BD, Chica RA, Mayo SL & Olafson BD* (2018) ProtaBank: A Repository for Protein Design and Engineering Data. Protein Science 27, 1113-1124.
Preprint available on bioRxiv.

St-Jacques AD, Gagnon O & Chica RA* (2018) Computational Enzyme Design: Successes, Challenges and Future Directions. In: Williams G & Hall M (Ed.) Modern Biocatalysis: Advances Towards Synthetic Biological Systems (pp. 88-116). Cambridge, UK. Royal Society of Chemistry.

Walton CJW, Parmeggiani F, Barber JEB, McCann JL, Turner NJ & Chica RA* (2018) Engineered Aminotransferase for the Production of D-Phenylalanine Derivatives using Biocatalytic Cascades. ChemCatChem 10, 470-474.


Davey JA, Damry AM, Goto NK* & Chica RA* (2017) Rational Design of Proteins that Exchange on Functional Timescales. Nature Chemical Biology 13, 1280-1285.
Access the recommendation on F1000

Chica RA* & Goto NK (2017) Rational Design of Protein Dynamics: Making Proteins Dance. Cell Systems, 5: 428–430. Invited commentary article.

Eason MG, Damry AM & Chica RA* (2017) Structure-Guided Rational Design of Red Fluorescent Proteins: Towards Designer Genetically-Encoded Fluorophores. Current Opinion in Structural Biology 45, 91-99.

Davey JA & Chica RA* (2017) Multistate Computational Protein Design with Backbone Ensembles. Methods in Molecular Biology 1529, 161-179.


Davey JA & Chica RA* (2016) Turning a Negative into a Positive: Conversion of a Homodimer into a Heterodimer using Negative State Repertoires. Structure 24, 496-497. Invited commentary article.

Pandelieva AT, Baran MJ, Calderini GF, McCann JL, Tremblay V, Sarvan S, Davey JA, Couture JF* & Chica RA* (2016) Brighter Red Fluorescent Proteins by Rational Design of Triple-Decker Motif. ACS Chemical Biology 11, 508-517.

St-Jacques AD, Rachel NM, Curry DR, Gillet SMFG, Clouthier CM, Keillor JW, Pelletier JN & Chica RA* (2016) Specificity of Transglutaminase-Catalyzed Peptide Synthesis. Journal of Molecular Catalysis B: Enzymatic 123, 53-61.


Davey JA, Damry AM, Euler CK, Goto NK & Chica RA* (2015) Prediction of Stable Globular Proteins using Negative Design With Non-Native Backbone Ensembles. Structure 23, 2011-2021.

Chica RA* (2015) Protein Engineering in the 21st Century. Protein Science 24, 431-433. Commentary article.

Davey JA & Chica RA* (2015) Optimization of Rotamers Prior to Template Minimization Improves Stability Predictions Made by Computational Protein Design. Protein Science 24, 545-560.

Lanouette S, Davey JA, Elisma F, Ning Z, Figeys D, Chica RA* & Couture JF* (2015) Discovery of Substrates for a SET Domain Lysine Methyltransferase Predicted by Multistate Computational Protein Design. Structure 23, 206-215.
Commentary on this article can be found here.


Barber JEB, Damry AM, Calderini GF, Walton CJW & Chica RA* (2014) Continuous Colorimetric Screening Assay for Detection of D-Amino Acid Aminotransferase Mutants Displaying Altered Substrate Specificity. Analytical Biochemistry 463, 23-30.

Davey JA & Chica RA* (2014) Improving the Accuracy of Protein Stability Predictions With Multistate Design Using a Variety of Backbone Ensembles. Proteins: Structure, Function and Bioinformatics 82, 771-784.


Mironov GG, St.-Jacques AD, Mungham A, Eason MG, Chica RA* & Berezovski MV* (2013) Bioanalysis for Biocatalysis: Multiplexed Capillary Electrophoresis–Mass Spectrometry Assay for Aminotransferase Substrate Discovery and Specificity Profiling. Journal of the American Chemical Society 135, 13728-13736.

Walton CJW & Chica RA* (2013) A High-Throughput Assay for Screening L- or D-amino Acid Specific Aminotransferase Mutant Libraries. Analytical Biochemistry 441, 190–198.


Moore MM, Oteng-Pabi SK, Pandelieva AT, Mayo SL & Chica RA* (2012) Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency through Rational Design. PLoS One 7: e52463.

Davey JA & Chica RA* (2012) Multistate Approaches in Computational Protein Design. Protein Science 21, 1241-1252.

Postdoctoral Research

Privett HK, Kiss G, Lee TM, Blomberg R, Chica RA, Thomas LM, Hilvert D, Houk KN & Mayo SL (2012) Iterative Approach to Computational Enzyme Design. Proceedings of the National Academy of Science of the USA 109, 3790-3795.

Hemmert AC, Otto TC, Chica RA, Wierdl M, Edwards JS, Lewis SL, Edwards CC, Tsurkan L, Cadieux CL, Kasten SA, Cashman JR, Mayo SL, Potter PM, Cerasoli DM & Redinbo MR (2011) Nerve Agent Hydrolysis Activity Designed into a Human Drug Metabolism Enzyme. PLoS One 6, e17441.

Chica RA, Moore MM, Allen BD & Mayo SL (2010) Generation of Longer Emission Wavelength Red Fluorescent Proteins Using Computationally Designed Libraries. Proceedings of the National Academy of Science of the USA 107, 20257-20262.
Access the recommendation on F1000

Graduate Research

Pardin C, Roy I, Chica RA, Bonneil E, Thibault P, Lubell WD, Pelletier JN & Keillor JW (2009) Photolabeling of Tissue Transglutaminase Reveals the Binding Mode of Potent Cinnamoyl Inhibitors. Biochemistry 48, 3346-53.

Keillor JW, Chica RA, Chabot N, Vinci V, Pardin C, Fortin E, Gillet SMFG, Nakano Y, Kaartinen MT, Pelletier JN & Lubell WD (2008) The Bioorganic Chemistry of Transglutaminase: From Mechanism to Inhibition and Engineering. Canadian Journal of Chemistry 86, 271-276.

Chica RA, Doucet N & Pelletier JN (2005) Semi-rational Approaches to Engineering Enzyme Activity: Combining the Benefits of Directed Evolution and Rational Design. Current Opinion in Biotechnology 16, 378-384.

Chica RA, Gagnon P, Keillor JW & Pelletier JN (2004) Tissue Transglutaminase Acylation: Proposed Role of Conserved Active Site Tyr and Trp Residues Revealed by Molecular Modelling of Peptide Substrate Binding. Protein Science 13, 979-991.

Gillet SMFG, Chica RA, Keillor JW & Pelletier JN (2004) Expression and Rapid Purification of Highly Active Hexahistidine-Tagged Guinea Pig Liver Transglutaminase. Protein Expression & Purification 33, 256-264.