{"id":64,"date":"2019-04-06T16:02:29","date_gmt":"2019-04-06T20:02:29","guid":{"rendered":"https:\/\/mysite.science.uottawa.ca\/lramunno\/?page_id=64"},"modified":"2019-04-30T17:06:52","modified_gmt":"2019-04-30T21:06:52","slug":"intense-laser-matter-interaction","status":"publish","type":"page","link":"https:\/\/mysite.science.uottawa.ca\/lramunno\/?page_id=64","title":{"rendered":"Nonlinear optical microscopy"},"content":{"rendered":"\n<p><strong> Effect of linear index mismatch and near fields :<\/strong><\/p>\n\n\n\n<p>Nonlinear optical  microscopes allow for label-free, molecule-specific,  non-destructive imaging of biological processes in cells and tissues.  Two such techniques are stimulated Raman scattering (SRS) microscopy  and coherent anti-Stokes Raman scattering (CARS) microscopy, which allow  for video-rate and hyperspectral imaging via molecular Raman resonances. <\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/mysite.science.uottawa.ca\/lramunno\/wp-content\/uploads\/2019\/04\/cars-srs-excerpt-1.jpg\" alt=\"\" class=\"wp-image-398\" width=\"272\" height=\"171\" srcset=\"https:\/\/mysite.science.uottawa.ca\/lramunno\/wp-content\/uploads\/2019\/04\/cars-srs-excerpt-1.jpg 503w, https:\/\/mysite.science.uottawa.ca\/lramunno\/wp-content\/uploads\/2019\/04\/cars-srs-excerpt-1-300x189.jpg 300w\" sizes=\"auto, (max-width: 272px) 100vw, 272px\" \/><\/figure><\/div>\n\n\n\n<p>We show in <a rel=\"noreferrer noopener\" aria-label=\"Optics Express (opens in a new tab)\" href=\"https:\/\/www.osapublishing.org\/oe\/abstract.cfm?uri=oe-24-22-25752\" target=\"_blank\">Optics Express<\/a>  (2016) that an inhomogeneous linear refractive index profile, such as that occurring  in biological tissues, is shown to significantly alter SRS and CARS images. In fact, near-field enhancement and microlensing can increase an object\u2019s perceived molecular density by almost an order of magnitude and can change its perceived position by up to 1.0 \u03bcm. Furthermore false CARS and AM-SRS signals can be observed in the absence of Raman active media. <\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/mysite.science.uottawa.ca\/lramunno\/wp-content\/uploads\/2019\/04\/Picture3.png\" alt=\"\" class=\"wp-image-391\" width=\"273\" height=\"134\" srcset=\"https:\/\/mysite.science.uottawa.ca\/lramunno\/wp-content\/uploads\/2019\/04\/Picture3.png 808w, https:\/\/mysite.science.uottawa.ca\/lramunno\/wp-content\/uploads\/2019\/04\/Picture3-300x146.png 300w, https:\/\/mysite.science.uottawa.ca\/lramunno\/wp-content\/uploads\/2019\/04\/Picture3-768x374.png 768w\" sizes=\"auto, (max-width: 273px) 100vw, 273px\" \/><\/figure><\/div>\n\n\n\n<p>Nonlinear optical imaging using backward propagating signals is very sensitive to the location of objects along the laser axis, and thus are used to image very small features. In our <a rel=\"noreferrer noopener\" aria-label=\" (opens in a new tab)\" href=\"https:\/\/www.osapublishing.org\/ol\/abstract.cfm?uri=ol-43-20-5082\" target=\"_blank\">Optics Letters<\/a> (2018), we show both theoretically and experimentally that the inhomogeneous refractive index in tendon  creates hot or cold spots in the constituent collagen fibrils, which can change the forward to backward signal ratio of the second harmonic generation (SHG) signal by up to 25%. This is caused by the extreme sensitivity of the backward signal on phase matching, which alters the destructive interference condition.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Effect of linear index mismatch and near fields : Nonlinear optical microscopes allow for label-free, molecule-specific, non-destructive imaging of biological processes in cells and tissues. Two such techniques are stimulated Raman scattering (SRS) microscopy and coherent anti-Stokes Raman scattering (CARS) microscopy, which allow for video-rate and hyperspectral imaging via molecular Raman resonances. We show in &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/mysite.science.uottawa.ca\/lramunno\/?page_id=64\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Nonlinear optical microscopy&#8221;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":33,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-64","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Nonlinear optical microscopy - Computational nanophotonics lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mysite.science.uottawa.ca\/lramunno\/?page_id=64\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nonlinear optical microscopy - Computational nanophotonics lab\" \/>\n<meta property=\"og:description\" content=\"Effect of linear index mismatch and near fields : Nonlinear optical microscopes allow for label-free, molecule-specific, non-destructive imaging of biological processes in cells and tissues. Two such techniques are stimulated Raman scattering (SRS) microscopy and coherent anti-Stokes Raman scattering (CARS) microscopy, which allow for video-rate and hyperspectral imaging via molecular Raman resonances. 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Two such techniques are stimulated Raman scattering (SRS) microscopy and coherent anti-Stokes Raman scattering (CARS) microscopy, which allow for video-rate and hyperspectral imaging via molecular Raman resonances. 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